{"product_id":"recombinant-cynomolgus-sialic-acid-binding-ig-like-lectin-15-siglec-15-cd33l3-c-6his-pksq050096","title":"Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15\/Siglec-15\/CD33L3 (C-6His) - PKSQ050096","description":"\u003cp\u003eRecombinant Cynomolgus Sialic acid-binding Ig-like lectin 15\/Siglec-15\/CD33L3 (C-6His)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSQ050096-10, PKSQ050096-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Siglec-15; CD33L3\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Angiopoietin-related protein 4; 425O18-1; Angiopoietin-like protein 4; Fasting-induced adipose factor; Hepatic fibrinogen\/angiopoietin-related protein; HFARP; Secreted protein Bk89; Angptl4; Farp; Fiaf; Ng27\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Cynomolgus macaques\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: A0A2K5UY47\u003c\/p\u003e\n\n\u003cp\u003eAccession: A0A2K5UY47\u003c\/p\u003e\n\n\u003cp\u003eBackground: Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO?-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE\/RANK Ligand signaling in osteoclasts.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized Cynomolgus Siglec-15-His(Cat#PKSQ050096) at 2μg\/ml (100 μl\/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 125 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Phe20-Thr263\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, 150mM NaCl, 0.3% Chaps, 5% Trehalose, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 27.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 30-40 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118130888883,"sku":"PKSQ050096-10","price":184.8,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118130954419,"sku":"PKSQ050096-50","price":550.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_614368ff-3906-4a23-90a4-ef05ae8519c5.png?v=1709636783","url":"https:\/\/afsbio.com\/products\/recombinant-cynomolgus-sialic-acid-binding-ig-like-lectin-15-siglec-15-cd33l3-c-6his-pksq050096","provider":"AFSBio Inc.","version":"1.0","type":"link"}