{"product_id":"recombinant-e-coli-tryptophan-synthase-β-chain-trp-b-protein-his-tag-pksq050055","title":"Recombinant E.coli Tryptophan Synthase β Chain\/Trp B Protein (His Tag) - PKSQ050055","description":"\u003cp\u003eRecombinant E.coli Tryptophan Synthase β Chain\/Trp B Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSQ050055-10, PKSQ050055-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Tryptophan Synthase β Chain; Trp B\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Tryptophan synthase beta chain; trpB\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P0A879\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: E.coli\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P0A879\u003c\/p\u003e\n\n\u003cp\u003eBackground: Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Thr2-Ile397\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 43.8 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 40-50 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43072228098227,"sku":"PKSQ050055-10","price":385.0,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43072228130995,"sku":"PKSQ050055-50","price":735.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_e56a77e6-d764-4725-92a0-4d8789841d93.png?v=1708684688","url":"https:\/\/afsbio.com\/products\/recombinant-e-coli-tryptophan-synthase-%ce%b2-chain-trp-b-protein-his-tag-pksq050055","provider":"AFSBio Inc.","version":"1.0","type":"link"}