{"product_id":"recombinant-enterokinase-his-bovine-bk0338","title":"Recombinant Enterokinase, His,Bovine - BK0338","description":"Recombinant Enterokinase, His,Bovine\n\nCatalogue Numbers: BK0338-100, BK0338-500\n\nSizes: 100IU, 500IU\n\nSource: P. pastoris\n\nMolecular Weight: Theoretical MW: 22.7 kDa.Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.\n\nPurity: \u0026gt; 95% by SDS-PAGE analyses.\n\nBiological Activity: 5 IU\/μl.\n Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.\n\nPhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\n\nFormulation: Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl\u003csub\u003e2\u003c\/sub\u003e, 50% glycerol, pH 7.4.\n\nAA Sequence: 0\n\nEndotoxin: \u0026lt; 1.0 EU\/μg, determined by LAL method.\n\nReconstitution: Reconstituted in ddH2O or PBS at 100 μg\/ml.\n\nStorage: Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20°C from date of receipt. It will remain stable at 37°C for one week without losing any activity.Please avoid freeze-thaw cycles.\n\nUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\n\nDescription: Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript.","brand":"BioWorld","offers":[{"title":"100IU","offer_id":42882264662195,"sku":"BK0338-100","price":217.41,"currency_code":"CAD","in_stock":true},{"title":"500IU","offer_id":42882264694963,"sku":"BK0338-500","price":691.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_88858a55-7e9b-440f-96ad-29358fdb2314.jpg?v=1702050342","url":"https:\/\/afsbio.com\/products\/recombinant-enterokinase-his-bovine-bk0338","provider":"AFSBio Inc.","version":"1.0","type":"link"}