{"product_id":"recombinant-hcc-4-ccl16-human-cho-expre-ssed-bk0219","title":"Recombinant HCC‑4\/CCL16, Human (CHO-expressed) - BK0219","description":"\u003cp\u003eRecombinant HCC‑4\/CCL16, Human (CHO-expre Ssed)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0219-10, BK0219-50\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eSource: CHO\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 12 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 98% as analyzed by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: The EC50 value of human HCC‑4\/CCL16 on Caˆ2+ mobilization assay in CHO-K1\/Ga15\/hCCR1 cells (human Ga15 and human CCR1 stably expressed in CHO-K1 cells) is less than 1.5 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against PBS.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: QPKVPEWVNTPSTCCLKYYEKVLPRRLVVGYRKALNCHLPAIIFVTKRNREVCTNPNDDWVQEYIKDPNLPLLPTRNLST\u003cbr\u003e\n VKIITAKNGQPQLLNSQ\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in ddH2O or PBS at 100 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant human HCC‑4\/CCL16 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human HCC‑4\/CCL16 should be stable up to 1 week at 4°C or up to 2 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Human HCC­4, also named NCC­4and Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1). It can signal through the CCR8 and CCR1 receptors, and it is chemotactic towards monocytes and lymphocytes but not neutrophils. HCC-4 is expressed weakly by some lymphocytes, including NK cells, T cells, and some T cell clones. The expression of HCC-4 in monocytes is greatly up-regulated in the presence of IL-10. HCC-4 induces a calcium flux in thp-1 cells that are desensitized prior to the expression of RANTES. Recombinant human HCC‑4\/CCL16 produced in CHO cells is a single non-glycosylated polypeptide chain containing 97 amino acids. A fully biologically active molecule, rhHCC‑4\/CCL16has a molecular mass of 12 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"10μg","offer_id":42882251882675,"sku":"BK0219-10","price":217.41,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":42882251915443,"sku":"BK0219-50","price":691.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_fbee162b-b9ea-4dec-919f-02ba393f4f24.jpg?v=1702050099","url":"https:\/\/afsbio.com\/products\/recombinant-hcc-4-ccl16-human-cho-expre-ssed-bk0219","provider":"AFSBio Inc.","version":"1.0","type":"link"}