{"product_id":"recombinant-human-alcam-cd166-protein-his-tagactive-pksh031919","title":"Recombinant Human ALCAM\/CD166 Protein (His Tag)(Active) - PKSH031919","description":"\u003cp\u003eRecombinant Human ALCAM\/CD166 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031919-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: ALCAM; CD166\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: CD166 antigen; Activated leukocyte cell adhesion molecule; CD166; ALCAM; MEMD\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Tags \u0026amp; Cell Markers; immunology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_001618.2\u003c\/p\u003e\n\n\u003cp\u003eBackground: Activated leukocyte cell adhesion molecule (ALCAM)\/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM\/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and\/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized human ALCAM-His at 10 μg\/ml (100 μl\/well) can bind mouse CD6-Fc, The EC50 of mouse CD6-Fc is 0.1-0.22 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Ala 526\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 98 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 57.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 60-70 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104330383539,"sku":"PKSH031919-100","price":680.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_fbf9d14d-92f3-4b39-acc0-b432eb28ca72.png?v=1709294480","url":"https:\/\/afsbio.com\/products\/recombinant-human-alcam-cd166-protein-his-tagactive-pksh031919","provider":"AFSBio Inc.","version":"1.0","type":"link"}