{"product_id":"recombinant-human-alkaline-phosphatase-alpi-protein-fc-tagactive-pksh030691","title":"Recombinant Human Alkaline Phosphatase\/ALPI Protein (Fc Tag)(Active) - PKSH030691","description":"\u003cp\u003eRecombinant Human Alkaline Phosphatase\/ALPI Protein (Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030691-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Alkaline Phosphatase; ALPI\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: IAP\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Tags \u0026amp; Cell Markers; Cell biology; Cancer; immunology; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-hFc\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P09923\u003c\/p\u003e\n\n\u003cp\u003eAccession: P09923\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interferon-alpha\/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases; which in turn phosphorylate several proteins; including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due; at least in part; to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1; in which Tyr466 is changed to phenylalanine; can act in a dominant negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is \u0026gt; 10, 000 pmoles\/min\/μg.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Asp 503\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 83 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 79.5 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 90-95 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107257483443,"sku":"PKSH030691-50","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_99febbd4-7ecc-4960-b226-45bf45147715.png?v=1709382048","url":"https:\/\/afsbio.com\/products\/recombinant-human-alkaline-phosphatase-alpi-protein-fc-tagactive-pksh030691","provider":"AFSBio Inc.","version":"1.0","type":"link"}