{"product_id":"recombinant-human-alkaline-phosphatase-alpi-protein-his-tagactive-pksh030690","title":"Recombinant Human Alkaline Phosphatase\/ALPI Protein (His Tag)(Active) - PKSH030690","description":"\u003cp\u003eRecombinant Human Alkaline Phosphatase\/ALPI Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030690-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Alkaline Phosphatase; ALPI\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: IAP\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Tags \u0026amp; Cell Markers; Cell biology; Cancer; immunology; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P09923\u003c\/p\u003e\n\n\u003cp\u003eAccession: P09923\u003c\/p\u003e\n\n\u003cp\u003eBackground: ALPI encodes for intestinal phosphatase alkaline; a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is \u0026gt; 1000 pmoles\/min\/μg.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Asp 503\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 54 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 66 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107257221299,"sku":"PKSH030690-50","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_487e63d8-3e21-4d29-a80d-6a8d5475db9e.png?v=1709382046","url":"https:\/\/afsbio.com\/products\/recombinant-human-alkaline-phosphatase-alpi-protein-his-tagactive-pksh030690","provider":"AFSBio Inc.","version":"1.0","type":"link"}