{"product_id":"recombinant-human-alkaline-phosphatase-alpl-protein-his-tag-pksh032057","title":"Recombinant Human Alkaline Phosphatase\/ALPL Protein (His Tag) - PKSH032057","description":"\u003cp\u003eRecombinant Human Alkaline Phosphatase\/ALPL Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032057-10, PKSH032057-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Alkaline Phosphatase; ALPL\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Alkaline Phosphatase; Tissue-Nonspecific Isozyme; AP-TNAP; TNSALP; Alkaline Phosphatase Liver\/Bone\/Kidney Isozyme; ALPL; HOPS; TNAP\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P05186\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Tags \u0026amp; Cell Markers; Cell biology; Cancer\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P05186\u003c\/p\u003e\n\n\u003cp\u003eBackground: Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) is a cell membrane protein which belongs to the alkaline phosphatase family. There are at least four distinct but related alkaline phosphatases in humans: intestinal AP (IAP), placental AP(PLAP), germ cell AP (GCAP) and their genes are clustered on chromosome 2, tissue-nonspecific isozyme (TNAP) which gene is located on chromosome 1. Alkaline phosphatases (APs) are dimeric enzymes, it catalyze the hydrolysis of phosphomonoesters with release of inorganic phosphate. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. This isozyme may play a role in skeletal mineralization. Mutations in ALPL gene have been linked directly to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones, and this disorder can vary depending on the specific mutation since this determines age of onset and severity of symptoms.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Leu18-Ser502\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2, pH 7.5.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 54.5 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 66 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43083652497587,"sku":"PKSH032057-10","price":312.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43083652530355,"sku":"PKSH032057-50","price":830.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_115c26fa-8934-4006-8a26-94c39f5c0007.png?v=1708946264","url":"https:\/\/afsbio.com\/products\/recombinant-human-alkaline-phosphatase-alpl-protein-his-tag-pksh032057","provider":"AFSBio Inc.","version":"1.0","type":"link"}