{"product_id":"recombinant-human-alkaline-phosphatase-intestinal-type-alpi-protein-rp00679","title":"Recombinant Human Alkaline phosphatase (Intestinal type)\/ALPI Protein - RP00679","description":"\u003cp\u003eRecombinant Human Alkaline phosphatase (Intestinal type)\/ALPI Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20ug, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00679-20, RP00679-50, RP00679-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Alkaline phosphatase (Intestinal type)\/ALPI Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val20-Thr502) of Human Alkaline phosphatase (Intestinal type)\/ALPI (Accession #NP_001622.2) fused with His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: ALPI, Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, EC:3.1.3.1\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P09923\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 248\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 90 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Recombinant Human Alkaline phosphatase (Intestinal type)\/ALPI Protein encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: VIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPACTT\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"20ug","offer_id":45198377681075,"sku":"RP00679-20","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198377713843,"sku":"RP00679-50","price":315.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198377746611,"sku":"RP00679-100","price":504.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_d2237788-f3f6-46ea-8935-c97346692c0c.png?v=1766961441","url":"https:\/\/afsbio.com\/products\/recombinant-human-alkaline-phosphatase-intestinal-type-alpi-protein-rp00679","provider":"AFSBio Inc.","version":"1.0","type":"link"}