{"product_id":"recombinant-human-anpep-cd13-protein-603-met-ile-his-tagactive-pksh031912","title":"Recombinant Human ANPEP\/CD13 Protein (603 Met\/Ile, His Tag)(Active) - PKSH031912","description":"\u003cp\u003eRecombinant Human ANPEP\/CD13 Protein (603 Met\/Ile, His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031912-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: ANPEP; CD13\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: APN; CD13; GP150; LAP1; P150; PEPN\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: immunology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_001141.2\u003c\/p\u003e\n\n\u003cp\u003eBackground: Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13\/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS\/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is \u0026gt; 2, 500 pmoles\/min\/μg.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Lys 69-Lys 967，603 Met\/Ile\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 97 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.3\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 104.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 135-140 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104330285235,"sku":"PKSH031912-50","price":1390.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_6c9bbef0-61e5-4c32-a100-b0b48663c6cf.png?v=1709294477","url":"https:\/\/afsbio.com\/products\/recombinant-human-anpep-cd13-protein-603-met-ile-his-tagactive-pksh031912","provider":"AFSBio Inc.","version":"1.0","type":"link"}