{"product_id":"recombinant-human-atg10-protein-his-t7-tag-pksh032097","title":"Recombinant Human ATG10 Protein (His\u0026T7 Tag) - PKSH032097","description":"\u003cp\u003eRecombinant Human ATG10 Protein (His\u0026amp;T7 Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032097-10, PKSH032097-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: ATG10\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Ubiquitin-Like-Conjugating Enzyme ATG10; Autophagy-Related Protein 10; APG10-Like; ATG10\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q9H0Y0\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cell biology; Cardiovascular; Neuroscience; Cancer; metabolism\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q9H0Y0\u003c\/p\u003e\n\n\u003cp\u003eBackground: Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Thr190\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 24.5 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 30 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43083656102067,"sku":"PKSH032097-10","price":312.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43083656134835,"sku":"PKSH032097-50","price":830.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_4c11758f-659f-4a88-851a-f3bf3b04a33b.png?v=1708946360","url":"https:\/\/afsbio.com\/products\/recombinant-human-atg10-protein-his-t7-tag-pksh032097","provider":"AFSBio Inc.","version":"1.0","type":"link"}