{"product_id":"recombinant-human-atg5-protein-pksh032101","title":"Recombinant Human ATG5 Protein - PKSH032101","description":"\u003cp\u003eRecombinant Human ATG5 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032101-10, PKSH032101-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: ATG5\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Autophagy protein 5; APG5-like; Apoptosis-specific protein; APG5L; ASP; ATG5;\u003c\/p\u003e \n\n\u003cp\u003eUNIProt ID: Q9H1Y0\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q9H1Y0\u003c\/p\u003e\n\n\u003cp\u003eBackground: ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Asp275\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 0.2M NaCl, 1mM DTT, 40%glycerol, pH 8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 32.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 33 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43083656528051,"sku":"PKSH032101-10","price":385.0,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43083656560819,"sku":"PKSH032101-50","price":735.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_7f457860-ec4b-4e12-be72-7fd439f3eaf8.png?v=1708946370","url":"https:\/\/afsbio.com\/products\/recombinant-human-atg5-protein-pksh032101","provider":"AFSBio Inc.","version":"1.0","type":"link"}