{"product_id":"recombinant-human-b3gat3-protein-his-tag-pksh033257","title":"Recombinant Human B3GAT3 Protein (His Tag) - PKSH033257","description":"\u003cp\u003eRecombinant Human B3GAT3 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033257-10, PKSH033257-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: B3GAT3\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: B3GAT3; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3; Beta-1; 3-glucuronyltransferase 3; Glucuronosyltransferase I; GlcAT-I; GlcUAT-I; Gal beta-1; 3-Gal-R glucuronyltransferase; ;\u003c\/p\u003e \n\n\u003cp\u003eResearch Area: Signal Transduction; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: O94766\u003c\/p\u003e\n\n\u003cp\u003eAccession: O94766\u003c\/p\u003e\n\n\u003cp\u003eBackground: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) is an enzyme that in humans is encoded by the B3GAT3 gene, belongs to the glycosyltransferase 43 family. B3GAT3 is involved in a number of biological processes such as catalyzing the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans, forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate, gGlycosaminoglycans biosynthesis, transfering a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1, 3-Gal-beta-1, 4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans.It also plays a role in the biosynthesis of l2\/HNK-1 carbohydrate epitope on glycoproteins , hows strict specificity for Gal-beta-1, 3-Gal-beta-1, 4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc and stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Glu72-Val335\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaC, 2mM EDTA, 20% Glycerol, pH 8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 30.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 31-34 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43113938682035,"sku":"PKSH033257-10","price":385.0,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43113938714803,"sku":"PKSH033257-50","price":735.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_bc4df9c1-11b9-4be4-a575-783c4c409335.png?v=1709548937","url":"https:\/\/afsbio.com\/products\/recombinant-human-b3gat3-protein-his-tag-pksh033257","provider":"AFSBio Inc.","version":"1.0","type":"link"}