{"product_id":"recombinant-human-b7-h3-c-6his-ept290","title":"Recombinant Human B7-H3 (C-6His) - EPT290","description":"\u003cp\u003eRecombinant Human B7-H3 (C-6His)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: EPT290-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD276, also known as B7-H3, is a member of the B7 superfamily with signature IgV and IgG regions in extracellular domains. It is a type I transmembrane protein and shares 20–27% amino acid identity with other B7 family members. B7-H3 is involved in the activation of T lymphocytes, and regulates murine bone formation. It is also reported that B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is expressed on T-cells, natural killer cells, and antigen presenting cells, as well as some non-immune cells, such as osteoblasts, fibroblasts, fibroblast-like synoviocytes and epithelial cells. High expression of B7-H3 in tumor vasculature also correlates with poor survival in patients, suggesting that it may play a role in tumor cell migration.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CD276; B7H34Ig-B7-H3; B7-H3; B7 homolog 3; CD276 antigen; CD276 molecule; Costimulatory molecule\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human B7 Homolog 3 is produced by our Mammalian expression system and the target gene encoding Leu29-Thr461 is expressed with a 6His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q5ZPR3\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 47.3 KDa\u003c\/p\u003e\n\n\u003cp\u003eApparent Molecular Weight: 65-90 KDa, reducing conditions\u003c\/p\u003e\n\n\u003cp\u003eStorage Condition: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003ePurity: Greater than 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Immobilized Human B7-H3-His (Cat#CK62) at 2μg\/ml (100 μl\/well) can bind Anti-Human B7-H3 mAb (Cat#NC055 ) The ED50 of Anti-Human B7-H3 mAb (Cat#NC055 ) is 7.98 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eRedissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg\/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\n\n\u003cp\u003eDelivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"Default Title","offer_id":43211051925683,"sku":"EPT290-50","price":740.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_3c37bb26-6fd4-4550-84af-ef55464f911c.png?v=1767117926","url":"https:\/\/afsbio.com\/products\/recombinant-human-b7-h3-c-6his-ept290","provider":"AFSBio Inc.","version":"1.0","type":"link"}