{"product_id":"recombinant-human-b7-h4-vtcn1-protein-fc-tagactive-pksh031453","title":"Recombinant Human B7-H4\/VTCN1 Protein (Fc Tag)(Active) - PKSH031453","description":"\u003cp\u003eRecombinant Human B7-H4\/VTCN1 Protein (Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031453-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: B7-H4; VTCN1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: B7S1; B7x; Vtcn1; B7h.5; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1VCTN1; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-hFc\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q7Z7D3\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q7Z7D3-1\u003c\/p\u003e\n\n\u003cp\u003eBackground: V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X \/ VTCN1 \/ B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X \/ VTCN1 \/ B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X \/ VTCN1 \/ B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 \/ interleukin-6 and IL10 \/ interleukin-10 and inhibited by CSF2 \/ GM-CSF and IL4 \/ interleukin-4 on antigen-presenting cells.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to inhibit anti-CD3 antibody and anti-CD28 antibody induced IFNγ secretion in human T lymphocytes. The ED50 for this effect is typically 0.7-3. 5μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Phe29-Ala258\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 52.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 66-76 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104352370867,"sku":"PKSH031453-100","price":1316.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_2d7c31a7-0915-44c0-b8c9-9ec5db13d355.png?v=1709294995","url":"https:\/\/afsbio.com\/products\/recombinant-human-b7-h4-vtcn1-protein-fc-tagactive-pksh031453","provider":"AFSBio Inc.","version":"1.0","type":"link"}