{"product_id":"recombinant-human-b7-h6-ncr3lg1-protein-rp01356","title":"Recombinant Human B7-H6\/NCR3LG1 Protein - RP01356","description":"\u003cp\u003eRecombinant Human B7-H6\/NCR3LG1 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01356-10, RP01356-20, RP01356-50, RP01356-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human B7-H6\/NCR3LG1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp25-Ser262) of human B7-H6\/NCR3LG1 (Accession #NP_001189368.1) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: B7-H6, NCR3LG1, B7 Homolog 6, NCR3LG1\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q68D85\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 374383\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 90 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Natural cytotoxicity triggering receptor 3 ligand 1(B7-H6) is a glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its binding ability in a functional ELISA.Immobilized Human NCR3LG1 (Catalog: RP01356) at 2 μg\/mL (100 μL\/well) can bind Mouse NCR3 (Catalog: RP00179) with a linear range of 0.03-46 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: DLKVEMMAGGTQITPLNDNVTIFCNIFYSQPLNITSMGITWFWKSLTFDKEVKVFEFFGDHQEAFRPGAIVSPWRLKSGDASLRLPGIQLEEAGEYRCEVVVTPLKAQGTVQLEVVASPASRLLLDQVGMKENEDKYMCESSGFYPEAINITWEKQTQKFPHPIEISEDVITGPTIKNMDGTFNVTSCLKLNSSQEDPGTVYQCVVRHASLHTPLRSNFTLTAARHSLSETEKTDNFS\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198308999347,"sku":"RP01356-10","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198309032115,"sku":"RP01356-20","price":266.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198309064883,"sku":"RP01356-50","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198309097651,"sku":"RP01356-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_4342e4f5-1f66-4b5e-ac82-8fe3a645da1c.png?v=1766960437","url":"https:\/\/afsbio.com\/products\/recombinant-human-b7-h6-ncr3lg1-protein-rp01356","provider":"AFSBio Inc.","version":"1.0","type":"link"}