{"product_id":"recombinant-human-bdnf-protein-with-pro-peptideactive-pksh033559","title":"Recombinant Human BDNF Protein (with pro peptide)(Active) - PKSH033559","description":"\u003cp\u003eRecombinant Human BDNF Protein (with pro peptide)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033559-10, PKSH033559-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: BDNF\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Brain-Derived Neurotrophic Factor; BDNF; Abrineurin\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cardiovascular; Neuroscience; metabolism\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P23560\u003c\/p\u003e\n\n\u003cp\u003eAccession: P23560\u003c\/p\u003e\n\n\u003cp\u003eBackground: The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis; whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival; growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects; Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized Human pro-BDNF at 2ug\/ml(100 μl\/well) can bind Human TrkB-His(Cat: PKSH033579). The ED50 of Human pro-BDNF is 1. 4 ug\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ala19-Arg247(R125A, R127A, R128A)\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 100mM NaCl, 0.02% Tween 80, pH 6.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 25.6 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 28 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118156316851,"sku":"PKSH033559-10","price":180.6,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118156349619,"sku":"PKSH033559-50","price":525.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_af17ce3d-4a66-4791-a472-20748ace7289.png?v=1709637237","url":"https:\/\/afsbio.com\/products\/recombinant-human-bdnf-protein-with-pro-peptideactive-pksh033559","provider":"AFSBio Inc.","version":"1.0","type":"link"}