{"product_id":"recombinant-human-beta-defensin-4-rhubd-4-pr1010","title":"Recombinant Human beta-Defensin 4 (rHuBD-4) - PR1010","description":"\u003cp\u003eRecombinant Human beta-Defensin 4 (rHuBD-4)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR1010-5, PR1010-20\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5µg, 20µg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: Approximately 6.0 KDa, a single non-glycosylated polypeptide chain containing 50 amino acids.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;98% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Determined by its ability to chemoattract human monocytes using a concentration range of 0.1-100.0 ng\/ml, corresponding to a Specific Activity of ＞1 x 104 IU\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 130mM NaCl.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 1EU\/mg of rHuBD-4 as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China\u003c\/p\u003e\n\n\u003cp\u003eDescription: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four human β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney. In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"5µg","offer_id":42882223800499,"sku":"PR1010-5","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"20µg","offer_id":42882223833267,"sku":"PR1010-20","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3d32f3fb-ab7d-497c-baca-6c9f64e0e08b.jpg?v=1702049579","url":"https:\/\/afsbio.com\/products\/recombinant-human-beta-defensin-4-rhubd-4-pr1010","provider":"AFSBio Inc.","version":"1.0","type":"link"}