{"product_id":"recombinant-human-brd4-n-10his-flag-pksh033982","title":"Recombinant Human BRD4 (N-10His-Flag) - PKSH033982","description":"\u003cp\u003eRecombinant Human BRD4 (N-10His-Flag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033982-10, PKSH033982-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: BRD4\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: HUNK1; HUNK1bromodomain-containing protein 4; HUNKI; MCAP\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cancer; epigenetics and nuclear signal\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-10His-Flag\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: O60885\u003c\/p\u003e\n\n\u003cp\u003eAccession: O60885\u003c\/p\u003e\n\n\u003cp\u003eBackground: Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Glu49-Glu460\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, 200mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 49 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 55-60 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43114008477875,"sku":"PKSH033982-10","price":183.4,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43114008510643,"sku":"PKSH033982-50","price":550.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_80b35581-8fcd-4f77-8e45-8e3485861fa5.png?v=1709550142","url":"https:\/\/afsbio.com\/products\/recombinant-human-brd4-n-10his-flag-pksh033982","provider":"AFSBio Inc.","version":"1.0","type":"link"}