{"product_id":"recombinant-human-c-kit-cd117-protein-aa-540-972-his-gst-tag-pksh030938","title":"Recombinant Human c-KIT\/CD117 Protein (aa 540-972, His \u0026 GST Tag) - PKSH030938","description":"\u003cp\u003eRecombinant Human c-KIT\/CD117 Protein (aa 540-972, His \u0026amp; GST Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030938-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: c-KIT, CD117\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Mast\/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117, PBT\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P10721\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eAccession: P10721-2\u003c\/p\u003e\n\n\u003cp\u003eBackground: C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1\/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Thr 540-Val 972\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 92 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 3mM DTT\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 76.8 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 68 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"50μg","offer_id":43155072417971,"sku":"PKSH030938-50","price":680.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_2f1d2ad7-e28e-4c43-b284-0a184544b45d.png?v=1710438465","url":"https:\/\/afsbio.com\/products\/recombinant-human-c-kit-cd117-protein-aa-540-972-his-gst-tag-pksh030938","provider":"AFSBio Inc.","version":"1.0","type":"link"}