{"product_id":"recombinant-human-c-met-hgfr-protein-his-tagactive-pksh031482","title":"Recombinant Human c-MET\/HGFR Protein (His Tag)(Active) - PKSH031482","description":"\u003cp\u003eRecombinant Human c-MET\/HGFR Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031482-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: c-MET; HGFR\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: AUTS9; c-Met; DFNB97; HGFR; RCCP2\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cancer; epigenetics and nuclear signal;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_000236\u003c\/p\u003e\n\n\u003cp\u003eBackground: Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and\/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF\/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized recombinant human HGF at 10 μg\/ml (100 μl\/well) can bind biotinylated c-Met. The EC50 of biotinylated c-Met is 5. 28 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Thr 932\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 103 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 45 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104313704627,"sku":"PKSH031482-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_4b0f06bc-70aa-4622-83b0-3e54fcb9b630.png?v=1709294095","url":"https:\/\/afsbio.com\/products\/recombinant-human-c-met-hgfr-protein-his-tagactive-pksh031482","provider":"AFSBio Inc.","version":"1.0","type":"link"}