{"product_id":"recombinant-human-camk1g-camki-gamma-protein-his-gst-tag-pksh030332","title":"Recombinant Human CAMK1G\/CaMKI gamma Protein (His \u0026 GST Tag) - PKSH030332","description":"\u003cp\u003eRecombinant Human CAMK1G\/CaMKI gamma Protein (His \u0026amp; GST Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030332-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CAMK1G, CaMKI gamma\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: CLICK3, CLICKIII, dJ272L16.1, RP1-272L16.2, VWS1\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q96NX5\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q96NX5-1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin\/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3\/CaMKI\u0026amp;gamma, is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKI\u0026amp;gamma, membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKI\u0026amp;gamma, critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKI\u0026amp;gamma, specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKI\u0026amp;gamma, may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Met 476\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 85 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 81 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 75 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"50μg","offer_id":43155063242931,"sku":"PKSH030332-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_d08e1161-662d-45fd-98e7-09038f8aa06d.png?v=1710438236","url":"https:\/\/afsbio.com\/products\/recombinant-human-camk1g-camki-gamma-protein-his-gst-tag-pksh030332","provider":"AFSBio Inc.","version":"1.0","type":"link"}