{"product_id":"recombinant-human-carbonic-anhydrase-5b-ca5b-protein-his-tagactive-pksh030856","title":"Recombinant Human Carbonic Anhydrase 5B\/CA5B Protein (His Tag)(Active) - PKSH030856","description":"\u003cp\u003eRecombinant Human Carbonic Anhydrase 5B\/CA5B Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030856-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Carbonic Anhydrase 5B; CA5B\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Carbonic Anhydrase 5B Mitochondrial; Carbonate Dehydratase VB; Carbonic Anhydrase VB; CA-VB; CA5B\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Tags \u0026amp; Cell Markers; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q9Y2D0\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q9Y2D0\u003c\/p\u003e\n\n\u003cp\u003eBackground: Carbonic anhydrase 5B; also known as carbonate dehydratase VB; carbonic anhydrase VB; CA-VB and CA5B; is amember of the alpha-carbonic anhydrase family. The strongest expression of CA5B \/ CA-VB is in heart; pancreas; kidney; placenta; lung; and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes; including respiration; calcification; acid-base balance; bone resorption; and the formation of aqueous humor; cerebrospinal fluid; saliva; and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B \/ CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A \/ CA-VA. CA5B \/ CA-VB has a wider tissue distribution than CA5A \/ CA-VA; which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A \/ CA-VA is activated by histamine; L-adrenaline; L- and D-histidine; and L- and D-phenylalanine. It is inhibited by coumarins; sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). CA5B \/ CA-VB is inhibited by coumarins; sulfonamide derivatives such as acetazolamide (AZA); saccharin and Foscarnet (phosphonoformate trisodium salt).\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its esterase activity. The specific activity is \u0026gt; 150 pmoles\/min\/μg.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Cys 34-Pro 317\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 97 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 20mM Tris, 50mM NaCl, 0.05% Brij-35, pH 8.0\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 34 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 34 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107260072115,"sku":"PKSH030856-50","price":1009.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_73383448-eed6-45a0-9fed-6e3095a8a262.png?v=1709382058","url":"https:\/\/afsbio.com\/products\/recombinant-human-carbonic-anhydrase-5b-ca5b-protein-his-tagactive-pksh030856","provider":"AFSBio Inc.","version":"1.0","type":"link"}