{"product_id":"recombinant-human-carboxypeptidase-b1-cpb1-protein-his-tagactive-pksh031567","title":"Recombinant Human Carboxypeptidase B1\/CPB1 Protein (His Tag)(Active) - PKSH031567","description":"\u003cp\u003eRecombinant Human Carboxypeptidase B1\/CPB1 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031567-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Carboxypeptidase B1; CPB1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Carboxypeptidase B; Pancreas-Specific Protein; PASP; CPB1; CPB; PCPB\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_001862.2\u003c\/p\u003e\n\n\u003cp\u003eBackground: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg, as measured using the wavelength at 254 nm. The specific activity is \u0026gt; 10000 pmoles\/min\/μg.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Tyr 417\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 98 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 25mM MES, 0.1 M NaCl, pH 6.5\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 47 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 45 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104316915891,"sku":"PKSH031567-50","price":1213.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_f746ce6f-b463-4b37-adb4-e380c8c5b83a.png?v=1709294163","url":"https:\/\/afsbio.com\/products\/recombinant-human-carboxypeptidase-b1-cpb1-protein-his-tagactive-pksh031567","provider":"AFSBio Inc.","version":"1.0","type":"link"}