{"product_id":"recombinant-human-cd122-il-2rb-protein-active-pksh031478","title":"Recombinant Human CD122\/IL-2RB Protein (Active) - PKSH031478","description":"\u003cp\u003eRecombinant Human CD122\/IL-2RB Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031478-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD122; IL-2RB\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: CD122; IL15RB; P70-75\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; immunology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture; ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_000869.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122\/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha\/beta subunit heterodimer, while the high affinity form consists of an alpha\/beta\/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122\/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122\/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122\/IL-2RB plays a role in regulating normal lymphocyte development.\u003c\/p\u003e\n\n\u003cp\u003eActivity: 1. Immobilized human IL2Rb at 10 μg\/ml (100 μl\/well) can bind biotinylated human IL2, The EC50 of biotinylated human IL2 is 0.1-0.24 μg\/mL. \u003cbr\u003e\n2. Measured by its ability to inhibit IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng\/mL of recombinant human IL-15.The ED50 for this effect is 1-4μg\/mL.\u003c\/p\u003e \n\n\u003cp\u003eSequence: Met 1-Asp 239\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 25.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 40 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104313114803,"sku":"PKSH031478-100","price":1771.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_6dae6b76-216e-4497-a759-b88b20c1f3a9.png?v=1709294088","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd122-il-2rb-protein-active-pksh031478","provider":"AFSBio Inc.","version":"1.0","type":"link"}