{"product_id":"recombinant-human-cd16a-fcgr3a-protein-176-phe-his-avi-tag-biotinylatedactive-pksh030284","title":"Recombinant Human CD16a\/FCGR3A Protein (176 Phe, His\u0026AVI Tag), Biotinylated(Active) - PKSH030284","description":"\u003cp\u003eRecombinant Human CD16a\/FCGR3A Protein (176 Phe, His\u0026amp;AVI Tag), Biotinylated(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH030284-20, PKSH030284-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD16a; FCGR3A\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; CD16a Antigen; Fc-Gamma RIII-Alpha; Fc-Gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc Receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3; CD16; CD16A\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Biotinylated\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His \u0026amp; AVI\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P08637\u003c\/p\u003e\n\n\u003cp\u003eAccession: P08637-1\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Fc receptor with low affinity for IgG (FCGR3; or CD16) is encoded by 2 nearly identical genes; FCGR3A and FCGR3B; resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A; it is also known as CD16a; encodes a transmembrane protein expressed on activated monocytes\/macrophages; natural killer (NK) cells; and a subset of T cells.\u003cbr\u003e\nCD16a \/ FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a \/ FCGR3A also has a broader function. CD16a \/ FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells; independent of antibody binding. Cross-linking of CD16a \/ FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a \/ FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells; independent of antibody ligation.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized Human IgG1 at 10 μg\/ml (100 μl\/well) can bind Human CD16a. The EC50 of Human CD16a is 2. 4 - 5. 7 μg\/ml. 3. Labeling ratio of biotin to protein: 0.7-1.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Gln 208\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 25.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 48 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43114026500275,"sku":"PKSH030284-20","price":1029.0,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43114026533043,"sku":"PKSH030284-100","price":2363.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_f7301a7f-8a03-4a6c-84f8-a692a92c2867.png?v=1709550438","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd16a-fcgr3a-protein-176-phe-his-avi-tag-biotinylatedactive-pksh030284","provider":"AFSBio Inc.","version":"1.0","type":"link"}