{"product_id":"recombinant-human-cd16b-fcgr3b-protein-active-pksh031290","title":"Recombinant Human CD16b\/FCGR3B Protein (Active) - PKSH031290","description":"\u003cp\u003eRecombinant Human CD16b\/FCGR3B Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031290-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD16b; FCGR3B\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; FcR-10; IgG Fc receptor III-1; FCG3; FCGR3; CD16b and FCGR4B; FCRIII; FCRIIIb\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: BLI\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: O75015\u003c\/p\u003e\n\n\u003cp\u003eAccession: O75015-1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Carbohydrate sulfotransferase 15; also known as N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; GalNAc4S-6ST; B-cell RAG-associated gene protein; CHST15 and BRAG; is a single-pass type II membrane protein which belongs to thesulfotransferase 1 family. CHST15 \/ BRAG is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen; lymph node; tonsil; bone marrow and peripheral leukocytes. It is not expressed in T-cells. In pro-B; pre-B; and mature B-cell lines; it colocalizes with RAG1. CHST15 \/ BRAG is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4; 6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence; GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4); to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15 \/ BRAG may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and \/ or regulation of B-cell-specific RAG expression.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Using the Octet RED System, the affinity constant (Kd) of CD16b bound to Human IgG1 was 80nM.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 18-Gly 193\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 20 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 22 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104352174259,"sku":"PKSH031290-100","price":1674.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_72e5f65d-c885-4cae-ac38-0ca10118fe1b.png?v=1709294983","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd16b-fcgr3b-protein-active-pksh031290","provider":"AFSBio Inc.","version":"1.0","type":"link"}