{"product_id":"recombinant-human-cd27-tnfrsf7-protein-fc-tagactive-pksh031924","title":"Recombinant Human CD27\/TNFRSF7 Protein (Fc Tag)(Active) - PKSH031924","description":"\u003cp\u003eRecombinant Human CD27\/TNFRSF7 Protein (Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 200μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031924-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD27; TNFRSF7\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: S152; S152. LPFS2; S152.LPFS2; T14; TNFRSF7; Tp55\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cancer; immunology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-rFc\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P26842\u003c\/p\u003e\n\n\u003cp\u003eAccession: P26842\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD27; also known as TNFRSF7; is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage; and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer; CD27 has been shown to play roles in lymphoid proliferation; differentiation; and apoptosis.It has important role in generation of T cell immunity; and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule; the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity; and meanwhile; plays a key role in regulating B-cell differentiation; activation and immunoglobulin synthesis.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its binding ability in a functional ELISA. Immobilized human CD27-his at 10 μg\/mL (100 μl\/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 95-150 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Ile192\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 44.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 63 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107262070963,"sku":"PKSH031924-200","price":1694.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_8abeedbb-bc95-4739-a739-3deefe4f6180.png?v=1709382103","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd27-tnfrsf7-protein-fc-tagactive-pksh031924","provider":"AFSBio Inc.","version":"1.0","type":"link"}