{"product_id":"recombinant-human-cd3-ε-cd3e-protein-his-tag-pksh031324","title":"Recombinant Human CD3 ε\/CD3E Protein (His Tag) - PKSH031324","description":"Recombinant Human CD3 ε\/CD3E Protein (His Tag)\n\nSize: 50μg\n\nCatalogue Number: PKSH031324-50\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: CD3 ε; CD3E\n\nTarget Symptom: T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3\/Leu-4 Epsilon Chain; CD3e; CD3E; T3E; CD3 epsilon; IMD18\n\nResearch Area: immunology; Stem cells\n\nTarget Species: Human\n\nExpression Host: HEK293 Cells\n\nFusion Tag: C-His\n\nAccession: NP_000724.1\n\nBackground: T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha\/beta and gamma\/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naï ve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.\n\nSequence: Met 1-Asp 126\n\nPurity: \u0026gt; 80 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 13.2 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107362406579,"sku":"PKSH031324-50","price":856.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_634f4edd-30c6-45e4-bd4c-329b6b4d8504.png?v=1709382797","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd3-%ce%b5-cd3e-protein-his-tag-pksh031324","provider":"AFSBio Inc.","version":"1.0","type":"link"}