{"product_id":"recombinant-human-cd3-epsilon-cd3e-c-mfc-pksh033869","title":"Recombinant Human CD3 epsilon\/CD3E (C-mFC) - PKSH033869","description":"\u003cp\u003eRecombinant Human CD3 epsilon\/CD3E (C-mFC)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033869-10, PKSH033869-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD3 epsilon; CD3E\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3\/Leu-4 Epsilon Chain; CD3e; CD3E; T3E\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: immunology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-mFC\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P07766\u003c\/p\u003e\n\n\u003cp\u003eAccession: P07766\u003c\/p\u003e\n\n\u003cp\u003eBackground: T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR\/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR\/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized Human CD3E-mFc at 10μg\/ml (100 μl\/well) can bind Human Anti-CD3. The ED50 of Human CD3E-mFc is 9.4 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Gln22-Thr48\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 28.5 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 32-40 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118162018483,"sku":"PKSH033869-10","price":184.8,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118162051251,"sku":"PKSH033869-50","price":550.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_f0516664-ff36-46fd-9224-41d1a852c91f.png?v=1709637348","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd3-epsilon-cd3e-c-mfc-pksh033869","provider":"AFSBio Inc.","version":"1.0","type":"link"}