{"product_id":"recombinant-human-cd31-pecam1-protein-fc-tagactive-pksh030454","title":"Recombinant Human CD31\/PECAM1 Protein (Fc Tag)(Active) - PKSH030454","description":"Recombinant Human CD31\/PECAM1 Protein (Fc Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSH030454-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: CD31; PECAM1\n\nTarget Symptom: Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA; PECA1; CD31; PECAM1\n\nTarget Species: Human\n\nExpression Host: HEK293 Cells\n\nApplication: Cell culture; ELISA\n\nFusion Tag: C-hFc\n\nAccession: EAW94208.1\n\nBackground: The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin α vβ 3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.\n\nActivity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 10\u003csup\u003e4\u003c\/sup\u003e cells\/well are added to sCD31-Fc coated plates (1. 25μg\/mL, 100μL\/well) in the presence of 20ng\/mL PMA, approximately 20-45% will adhere after 30 minutes at 37℃.\n\nSequence: Met 1-Lys 601\n\nPurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 91.2 kDa\n\nObserved MW: 130-140 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104257441971,"sku":"PKSH030454-100","price":680.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_3841db32-684e-4c53-a825-d8fc5b55b41d.png?v=1709293128","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd31-pecam1-protein-fc-tagactive-pksh030454","provider":"AFSBio Inc.","version":"1.0","type":"link"}