{"product_id":"recombinant-human-cd31-pecam1-protein-his-tagactive-pksh031833","title":"Recombinant Human CD31\/PECAM1 Protein (His Tag)(Active) - PKSH031833","description":"Recombinant Human CD31\/PECAM1 Protein (His Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSH031833-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: CD31; PECAM1\n\nTarget Symptom: Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA; PECA1; CD31; PECAM1\n\nTarget Species: Human\n\nExpression Host: HEK293 Cells\n\nApplication: Cell culture; ELISA\n\nFusion Tag: C-His\n\nAccession: EAW94208.1\n\nBackground: The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin α vβ 3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.\n\nActivity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells\/well are added to CD31-His coated plates (5μg\/mL, 100 μL\/well) in the presence of 10 μg\/mL PHA, approximately \u0026gt; 70% will adhere after 30 minutes at 37℃.\n\nSequence: Met 1-Lys 601\n\nPurity: \u0026gt; 97 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 66 kDa\n\nObserved MW: 100-110 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104260128947,"sku":"PKSH031833-100","price":635.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_3e4ba4f6-adb7-4d7f-b7fb-0ca1a7f5915c.png?v=1709293241","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd31-pecam1-protein-his-tagactive-pksh031833","provider":"AFSBio Inc.","version":"1.0","type":"link"}