{"product_id":"recombinant-human-cd3d-cd3e-heterodimer-protein-active-pksh030479","title":"Recombinant Human CD3D \u0026 CD3E Heterodimer Protein (Active) - PKSH030479","description":"\u003cp\u003eRecombinant Human CD3D \u0026amp; CD3E Heterodimer Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030479-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD3D \u0026amp; CD3E Heterodimer\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: T-Cell Surface Glycoprotein CD3 Delta Chain; T-Cell Receptor T3 Delta Chain; CD3d; CD3D; T3D\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: immunology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-Flag \u0026amp; hFc\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P04234\u0026amp;P07766\u003c\/p\u003e\n\n\u003cp\u003eAccession: P04234-1\u0026amp;P07766-1\u003c\/p\u003e\n\n\u003cp\u003eBackground: T-cell surface glycoprotein CD3D \u0026amp; CD3E, also known as CD3 delta \u0026amp; CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha\/beta and gamma\/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its binding ability in a functional ELISA. Immobilized human CD3D \u0026amp; CD3E Heterodimer Protein at 10 μg\/ml (100 μl\/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Ala105\u0026amp;Met 1-Asp126\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 77.5 (37.5+40） kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 45-50 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107256533171,"sku":"PKSH030479-50","price":926.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_00d96bb6-668b-41f2-a0ec-ecc6d240f2cc.png?v=1709382027","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd3d-cd3e-heterodimer-protein-active-pksh030479","provider":"AFSBio Inc.","version":"1.0","type":"link"}