{"product_id":"recombinant-human-cd4-leu3-protein-aa-1-208-his-tagactive-pksh031631","title":"Recombinant Human CD4\/LEU3 Protein (aa 1-208, His Tag)(Active) - PKSH031631","description":"Recombinant Human CD4\/LEU3 Protein (aa 1-208, His Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSH031631-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: CD4; LEU3\n\nTarget Symptom: T-cell surface glycoprotein CD4; T-cell surface antigen T4\/Leu-3; CD4; Scd4; CD4mut\n\nResearch Area: immunology; Stem cells\n\nTarget Species: Human\n\nExpression Host: HEK293 Cells\n\nApplication: ELISA\n\nFusion Tag: C-His\n\nAccession: NP_000607.1\n\nBackground: T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper\/inducer subsets of T lymphocytes, has now been critically identified as the binding\/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4.\n\nActivity: 1. Measured by its ability to bind biotinylated GP140-His in a functional ELISA. 2. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When 5 x 10\u003csup\u003e4\u003c\/sup\u003e cells\/well are added to CD4-coated plates (2. 5 μg\/ml and 100 μl\/well), approximately 40 %-60 % will adhere specifically after 60 minutes at 37℃.\n\nSequence: Met 1-Ser 208\n\nPurity: \u0026gt; 94 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 21.7 kDa\n\nObserved MW: 26 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104319897779,"sku":"PKSH031631-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_7e154a04-1f4b-4378-832d-62fdd549bb7f.png?v=1709294220","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd4-leu3-protein-aa-1-208-his-tagactive-pksh031631","provider":"AFSBio Inc.","version":"1.0","type":"link"}