{"product_id":"recombinant-human-cd47-protein-rp01306","title":"Recombinant Human CD47 Protein - RP01306","description":"\u003cp\u003eRecombinant Human CD47 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: RP01306-50, RP01306-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human CD47 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln19-Pro139) of human CD47 (Accession #NP_001768.1) fused with an 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CD47, IAP, MER6, OA3, CD47 molecule, IAP, MER6, OA3\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q08722\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 961\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: This protein is a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 5 μg\/mL (100 μL\/well) can bind Human SIRPA with a linear range of 0.488-189.26 ng\/mL.|Measured by its binding ability in a functional ELISA. Immobilized Human CD47 (Catalog: RP01306) at 2 μg\/mL (100 μL\/well) can bind Human SIRP-alpha\/CD172a (Catalog: RP00171) with a linear range of 0.06-22.3 ng\/mL.|3.Loaded Human SIRP-alpha\/CD172a Protein, C-hFc\u0026amp;His(Catalog: RP00171) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (Catalog: RP01306) with an affinity constant of 11.1 nM as determined in BLI assay (Gator).\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"50ug","offer_id":45198361952435,"sku":"RP01306-50","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198361985203,"sku":"RP01306-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_c6ea96ec-6108-433d-8e8a-acb5ff4c438b.png?v=1766961191","url":"https:\/\/afsbio.com\/products\/recombinant-human-cd47-protein-rp01306","provider":"AFSBio Inc.","version":"1.0","type":"link"}