{"product_id":"recombinant-human-cdk2-protein-e-coli-his-tag-pdeh100009","title":"Recombinant Human CDK2 Protein (E.coli, His Tag) - PDEH100009","description":"\u003cp\u003eRecombinant Human CDK2 Protein (E.coli, His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PDEH100009-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CDK2\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Cyclin-Dependent Kinase 2; Cell Division Protein Kinase 2; p33 Protein Kinase; CDK2; CDKN2\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P24941\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P24941\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser\/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1\/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2\/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1\/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Leu298\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% tween-80.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 36.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 34 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43072302874803,"sku":"PDEH100009-100","price":165.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_2c10f73e-b50f-4163-a380-76d3fde9c43b.png?v=1708685335","url":"https:\/\/afsbio.com\/products\/recombinant-human-cdk2-protein-e-coli-his-tag-pdeh100009","provider":"AFSBio Inc.","version":"1.0","type":"link"}