{"product_id":"recombinant-human-cntf-protein-active-pksh033716","title":"Recombinant Human CNTF Protein (Active) - PKSH033716","description":"\u003cp\u003eRecombinant Human CNTF Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μg, 10μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033716-50, PKSH033716-10\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CNTF\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Ciliary Neurotrophic Factor; CNTF\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Neuroscience; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P26441\u003c\/p\u003e\n\n\u003cp\u003eAccession: P26441\u003c\/p\u003e\n\n\u003cp\u003eBackground: Ciliary Neurotrophic Factor (CNTF) is a potent survival factor for neurons and oligodendrocytes. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal transducing subunit in their receptor complexes. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways. The mechanism underlying the release of CNTF is unknown.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 25 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ala2-Met200\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, 1mM Cysteine, PH7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 22.93 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 25 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"50μg","offer_id":43118158971059,"sku":"PKSH033716-50","price":582.4,"currency_code":"CAD","in_stock":true},{"title":"10μg","offer_id":43118159003827,"sku":"PKSH033716-10","price":201.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_dea3be5f-1dfc-4600-967e-63e25d46e4dd.png?v=1709637292","url":"https:\/\/afsbio.com\/products\/recombinant-human-cntf-protein-active-pksh033716","provider":"AFSBio Inc.","version":"1.0","type":"link"}