{"product_id":"recombinant-human-collagen-alpha-1ichain-col1a1-protein-trx-his-tag-pdeh100132","title":"Recombinant Human Collagen alpha-1(I)chain\/COL1A1 protein (TRX,His tag) - PDEH100132","description":"\u003cp\u003eRecombinant Human Collagen alpha-1(I)chain\/COL1A1 protein (TRX,His tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PDEH100132-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Collagen alpha-1 chain; COL1A1\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Collagen alpha-1(I) chain; Alpha-1 type I collagen; COL1A1; COL1A1\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P02452\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cell biology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-Trx-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P02452\u003c\/p\u003e\n\n\u003cp\u003eBackground: Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Glu 1021-Gly 1109\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 29.68 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 32 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43072322470067,"sku":"PDEH100132-100","price":165.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_19a41ad0-0ca8-48d3-bedc-35961f7c6199.png?v=1708685453","url":"https:\/\/afsbio.com\/products\/recombinant-human-collagen-alpha-1ichain-col1a1-protein-trx-his-tag-pdeh100132","provider":"AFSBio Inc.","version":"1.0","type":"link"}