{"product_id":"recombinant-human-collagen-i-alpha-1-col1a1-protein-rp01886","title":"Recombinant Human Collagen I alpha 1\/COL1A1 Protein - RP01886","description":"\u003cp\u003eRecombinant Human Collagen I alpha 1\/COL1A1 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01886-10, RP01886-20, RP01886-50, RP01886-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Collagen I alpha 1\/COL1A1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp1219-Leu1464 ) of Human Collagen I alpha 1\/COL1A1 (Accession #NP_000079.2) fused with hFc tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P02452\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 1277\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95% as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-hFc\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: DDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWIDPNQGCNLDAIKVFCNMETGETCVYPTQPSVAQKNWYISKNPKDKRHVWFGESMTDGFQFEYGGQGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIEIRAEGNSRFTYSVTVDGCTSHTGAWGKTVIEYKTTKTSRLPIIDVAPLDVGAPDQEFGFDVGPVCFL\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.01 EU\/μg of the protein by LAL method\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198254407859,"sku":"RP01886-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198254440627,"sku":"RP01886-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198254473395,"sku":"RP01886-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198254506163,"sku":"RP01886-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_11fbbbb7-90b1-437e-a7d9-94cb3465389c.png?v=1766959777","url":"https:\/\/afsbio.com\/products\/recombinant-human-collagen-i-alpha-1-col1a1-protein-rp01886","provider":"AFSBio Inc.","version":"1.0","type":"link"}