{"product_id":"recombinant-human-contactin-3-cntn3-protein-708-asp-asn-fc-tagactive-pksh031803","title":"Recombinant Human Contactin 3\/CNTN3 Protein (708 Asp\/Asn, Fc Tag)(Active) - PKSH031803","description":"Recombinant Human Contactin 3\/CNTN3 Protein (708 Asp\/Asn, Fc Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSH031803-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: Contactin 3; CNTN3\n\nTarget Symptom: BIG-1; PANG; PCS\n\nResearch Area: Neuroscience\n\nTarget Species: Human\n\nExpression Host: HEK293 Cells\n\nApplication: Cell culture; ELISA\n\nFusion Tag: C-hFc\n\nAccession: NP_065923.1\n\nBackground: Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1; Contactin-2(TAG-1); Contactin-3(BIG-1); BIG-2; Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s; Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast; the function of the other four members of this subgroup remained unknown until recently. Contactin-3; also known as CNTN3 ( BIG-1 in rat and PANG in mouse ); is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells; amygdaloid and thalamic neurons and olfactory granule cells. In the brain; Contactin-3 is expressed in frontal lobe; occipital lobe; cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.\n\nActivity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10\u003csup\u003e4\u003c\/sup\u003e cells\/well are added to CNTN3 coated plates (0.8 μg\/ml and 100 μl\/well), approximately 30%-50% will adhere specifically after 60 minutes at 37℃.\n\nSequence: Met 1-Ser 1002; 708 Asp\/Asn\n\nPurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 134.5 kDa\n\nObserved MW: 160-170 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104325959859,"sku":"PKSH031803-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_c8d845a1-84a3-4fe4-b59f-90b735274d4f.png?v=1709294363","url":"https:\/\/afsbio.com\/products\/recombinant-human-contactin-3-cntn3-protein-708-asp-asn-fc-tagactive-pksh031803","provider":"AFSBio Inc.","version":"1.0","type":"link"}