{"product_id":"recombinant-human-cripto-1-tdgf1-protein-rp00202","title":"Recombinant Human Cripto-1\/TDGF1 Protein - RP00202","description":"\u003cp\u003eRecombinant Human Cripto-1\/TDGF1 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20ug, 50ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00202-10, RP00202-20, RP00202-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Cripto-1\/TDGF1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu31-Thr172) of human Cripto\/TDGF1 (Accession #NP_003203) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: TDGF1, CR, CRGF, CRIPTO\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P13385\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 6997\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cripto\/TDGF1 is a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family. TDGF1 is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. It is overexpressed in many types of cancers and acts as a growth factor for tumors. Cripto mutants display defects in mesoderm induction and heart morphogenesis, similar to phenotypes seen in Nodal mutants. Cripto can also activate mitogen-activated protein kinase (MAPK) and Akt pathways independently of Nodal by directly binding to a membrane-associated heparan sulfate proteoglycan, glypican-1.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Cripto at 2 μg\/mL (100 μL\/well) can bind Recombinant Human ALK4 with a linear range of 0.15-151 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: LGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPPMGIQHSKELNRTCCLNGGTCMLGSFCACPPSFYGRNCEHDVRKENCGSVPHDTWLPKKCSLCKCWHGQLRCFPQAFLPGCDGLVMDEHLVASRTPELPPSART\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198368014515,"sku":"RP00202-10","price":168.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198368047283,"sku":"RP00202-20","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198368080051,"sku":"RP00202-50","price":420.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_4228cdc9-1348-4f97-a551-8c8a19a996b4.png?v=1766961280","url":"https:\/\/afsbio.com\/products\/recombinant-human-cripto-1-tdgf1-protein-rp00202","provider":"AFSBio Inc.","version":"1.0","type":"link"}