{"product_id":"recombinant-human-cxadr-car-protein-his-fc-tagactive-pksh031425","title":"Recombinant Human CXADR\/CAR Protein (His \u0026 Fc Tag)(Active) - PKSH031425","description":"Recombinant Human CXADR\/CAR Protein (His \u0026amp; Fc Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSH031425-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: CXADR; CAR\n\nTarget Symptom: Coxsackievirus and Adenovirus Receptor; CAR; hCAR; CVB3-Binding Protein; Coxsackievirus B-Adenovirus Receptor; HCVADR; CXADR; CAR\n\nResearch Area: Signal Transduction; Microbiology; \n\nTarget Species: Human\n\nExpression Host: HEK293 Cells\n\nApplication: Cell culture; ELISA\n\nFusion Tag: C-His \u0026amp; Fc\n\nAccession: NP_001329.1\n\nBackground: CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which, D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.\n\nActivity: Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 5 x 10\u003csup\u003e4\u003c\/sup\u003e cells\/well are added to CXADR coated plates (4 μg\/ml and 100 μl\/well), approximately 20%-40% will adhere specifically after 60 minutes at 37℃.\n\nSequence: Met 1-Gly 237\n\nPurity: \u0026gt; 92 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 52 kDa\n\nObserved MW: 60-65 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104312066227,"sku":"PKSH031425-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_d88e83eb-52cf-4550-9bd0-e10e291a9100.png?v=1709294056","url":"https:\/\/afsbio.com\/products\/recombinant-human-cxadr-car-protein-his-fc-tagactive-pksh031425","provider":"AFSBio Inc.","version":"1.0","type":"link"}