{"product_id":"recombinant-human-cyclin-d1-ccnd1-protein-his-tag-pdeh100267","title":"Recombinant Human cyclin D1\/Ccnd1 protein (His tag) - PDEH100267","description":"\u003cp\u003eRecombinant Human cyclin D1\/Ccnd1 protein (His tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PDEH100267-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: cyclin D1; Ccnd1\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: B cell leukemia 1; BCL 1; ccnd1; Cd1; Cyl 1; G1\/S specific cyclin D1; PRAD1\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P25322\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cell Biology; Epigenetics and Nuclear Signaling; Cancer\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P25322\u003c\/p\u003e\n\n\u003cp\u003eBackground: Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip\/Kip or INK family of proteins. The inactive ternary complex of cyclin D\/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase. The active complex of cyclin D\/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1\/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Ile 295\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 32.34 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 39 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43083538366643,"sku":"PDEH100267-100","price":165.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_021f1b32-b0f5-4929-b2c7-bee3428baa39.png?v=1708944301","url":"https:\/\/afsbio.com\/products\/recombinant-human-cyclin-d1-ccnd1-protein-his-tag-pdeh100267","provider":"AFSBio Inc.","version":"1.0","type":"link"}