{"product_id":"recombinant-human-cyclin-d1-protein-his-tag-pdeh100238","title":"Recombinant Human Cyclin D1 protein (His tag) - PDEH100238","description":"\u003cp\u003eRecombinant Human Cyclin D1 protein (His tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PDEH100238-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Cyclin D1\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: B cell leukemia 1; BCL 1; ccnd1; Cd1; Cyl 1; G1\/S specific cyclin D1; PRAD1\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P24385\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cancer; Epigenetics and Nuclear Signaling; Cell Biology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P24385\u003c\/p\u003e\n\n\u003cp\u003eBackground: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1\/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB\/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1\/CDK4\/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ala 16-Ala 271\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 28.05 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 35 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43072326205619,"sku":"PDEH100238-100","price":165.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_0feb9a01-a000-4434-87db-ed5100123fe5.png?v=1708685652","url":"https:\/\/afsbio.com\/products\/recombinant-human-cyclin-d1-protein-his-tag-pdeh100238","provider":"AFSBio Inc.","version":"1.0","type":"link"}