{"product_id":"recombinant-human-cynomolgus-vegfa-vegf165-protein-active-pksh031978","title":"Recombinant Human\/Cynomolgus VEGFA\/VEGF165 Protein (Active) - PKSH031978","description":"\u003cp\u003eRecombinant Human\/Cynomolgus VEGFA\/VEGF165 Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH031978-20, PKSH031978-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Human; VEGFA; VEGF165\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: MVCD1; VEGF; VEGF165; VPF; Vascular Endothelial Growth Factor Isoform 165\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P15692\u003c\/p\u003e\n\n\u003cp\u003eAccession: P15692-4\u003c\/p\u003e\n\n\u003cp\u003eBackground: Vascular endothelial growth factor (VEGF); also known as vascular permeability factor (VPF) and VEGF-A; is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)\/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects; including mediating increased vascular permeability; inducing angiogenesis; vasculogenesis and endothelial cell growth; promoting cell migration; inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability; thus it was originally referred to as vascular permeability factor.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 4-16 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Arg191\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 19.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 20 and 22 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43114027712691,"sku":"PKSH031978-20","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43114027745459,"sku":"PKSH031978-100","price":1589.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_f83ae614-c9ea-4b2f-891d-16bf9aab0b98.png?v=1709550452","url":"https:\/\/afsbio.com\/products\/recombinant-human-cynomolgus-vegfa-vegf165-protein-active-pksh031978","provider":"AFSBio Inc.","version":"1.0","type":"link"}