{"product_id":"recombinant-human-cystatin-7-cst7-protein-fc-tagactive-pksh031813","title":"Recombinant Human Cystatin 7\/CST7 Protein (Fc Tag)(Active) - PKSH031813","description":"\u003cp\u003eRecombinant Human Cystatin 7\/CST7 Protein (Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 20μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031813-20\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Cystatin 7; CST7\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Cystatin-F; Cystatin-7; Cystatin-Like Metastasis-Associated Protein; CMAP; Leukocystatin; CST7\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cell biology; Cardiovascular\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-hFc\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_003641.3\u003c\/p\u003e\n\n\u003cp\u003eBackground: The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (R\u0026amp;D Systems, Catalog#ES008). The IC50 value is \u0026lt; 5 nM.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-His 145\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 97 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 41.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 50 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104326615219,"sku":"PKSH031813-20","price":856.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_6e437291-e29b-418d-87cf-13eabf05568b.png?v=1709294376","url":"https:\/\/afsbio.com\/products\/recombinant-human-cystatin-7-cst7-protein-fc-tagactive-pksh031813","provider":"AFSBio Inc.","version":"1.0","type":"link"}