{"product_id":"recombinant-human-dapk1-dap-kinase-1-protein-aa-1-363-his-gst-tagactive-pksh030343","title":"Recombinant Human DAPK1\/DAP Kinase 1 Protein (aa 1-363, His \u0026 GST Tag)(Active) - PKSH030343","description":"\u003cp\u003eRecombinant Human DAPK1\/DAP Kinase 1 Protein (aa 1-363, His \u0026amp; GST Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030343-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: DAPK1; DAP Kinase 1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: DAPK\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P53355\u003c\/p\u003e\n\n\u003cp\u003eAccession: P53355-1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to theprotein kinase superfamily, CAMK Ser \/ Thr protein kinase family and DAP kinase subfamily. DAPK1 contains tenANK repeats, onedeath domain and oneprotein kinase domain. DAPK1 is a calcium \/ calmodulin-dependent serine\/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).\u003c\/p\u003e\n\n\u003cp\u003eActivity: The specific activity was determined to be 20 nmol\/min\/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Leu 363\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 80 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 69.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 64 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104347652275,"sku":"PKSH030343-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_502aa8a0-f31c-4bbd-aa0e-c2feaa897b25.png?v=1709294877","url":"https:\/\/afsbio.com\/products\/recombinant-human-dapk1-dap-kinase-1-protein-aa-1-363-his-gst-tagactive-pksh030343","provider":"AFSBio Inc.","version":"1.0","type":"link"}