{"product_id":"recombinant-human-dcamkl1-protein-aa-1-705-his-gst-tagactive-pksh030359","title":"Recombinant Human DCAMKL1 Protein (aa 1-705, His \u0026 GST Tag)(Active) - PKSH030359","description":"\u003cp\u003eRecombinant Human DCAMKL1 Protein (aa 1-705, His \u0026amp; GST Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030359-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: DCAMKL1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: CL1; CLICK1; DCAMKL1; DCDC3A; DCLK\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: O15075\u003c\/p\u003e\n\n\u003cp\u003eAccession: O15075-1\u003c\/p\u003e\n\n\u003cp\u003eBackground: DCAMKL1; also known as DCLK1; is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains; which bind microtubules and regulate microtubule polymerization; a C-terminal serine\/threonine protein kinase domain; which shows substantial homology to Ca2+\/calmodulin-dependent protein kinase; and a serine\/proline-rich domain in between the doublecortin and the protein kinase domains; which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes; including neuronal migration; retrograde transport; neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.\u003c\/p\u003e\n\n\u003cp\u003eActivity: The specific activity was determined to be 6. 1 nmol\/min\/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Val 705\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 84 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 0.5mM PMSF\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 106 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 105 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104348307635,"sku":"PKSH030359-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_e92d83ea-838d-4e2d-a69e-46129340798d.png?v=1709294887","url":"https:\/\/afsbio.com\/products\/recombinant-human-dcamkl1-protein-aa-1-705-his-gst-tagactive-pksh030359","provider":"AFSBio Inc.","version":"1.0","type":"link"}