{"product_id":"recombinant-human-ddr1-kinase-mck10-protein-aa-444-913-his-gst-tagactive-pksh030389","title":"Recombinant Human DDR1 Kinase\/MCK10 Protein (aa 444-913, His \u0026 GST Tag)(Active) - PKSH030389","description":"\u003cp\u003eRecombinant Human DDR1 Kinase\/MCK10 Protein (aa 444-913, His \u0026amp; GST Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030389-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: DDR1 Kinase; MCK10\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: CAK; CD167; DDR; EDDR1; HGK2; MCK10; NEP; NTRK4; PTK3; PTK3A; RTK6; TRKE\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q08345\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q08345-1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1\/MCK10\/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1\/MCK10\/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.\u003c\/p\u003e\n\n\u003cp\u003eActivity: The specific activity was determined to be 2. 75 nmol\/min\/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Arg444-Val913\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 89 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 80 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 80 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104349094067,"sku":"PKSH030389-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_b63d5f94-fbc4-4b4c-a44b-a8d1025caa66.png?v=1709294908","url":"https:\/\/afsbio.com\/products\/recombinant-human-ddr1-kinase-mck10-protein-aa-444-913-his-gst-tagactive-pksh030389","provider":"AFSBio Inc.","version":"1.0","type":"link"}