{"product_id":"recombinant-human-ddr2-kinase-cd167b-protein-aa-422-855-his-gst-tagactive-pksh030420","title":"Recombinant Human DDR2 Kinase\/CD167b Protein (aa 422-855, His \u0026 GST Tag)(Active) - PKSH030420","description":"\u003cp\u003eRecombinant Human DDR2 Kinase\/CD167b Protein (aa 422-855, His \u0026amp; GST Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030420-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: DDR2 Kinase; CD167b\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: CD167; MIG20a; NTRKR3; TKT; TYRO10\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cardiovascular; Neuroscience\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q16832\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q16832\u003c\/p\u003e\n\n\u003cp\u003eBackground: Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2\/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2\/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2\/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2\/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.\u003c\/p\u003e\n\n\u003cp\u003eActivity: The specific activity was determined to be 8 nmol\/min\/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Arg422-Glu855\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 77.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 77 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107256467635,"sku":"PKSH030420-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_0067172e-22e7-4ffb-84ba-a10b80145c9a.png?v=1709382022","url":"https:\/\/afsbio.com\/products\/recombinant-human-ddr2-kinase-cd167b-protein-aa-422-855-his-gst-tagactive-pksh030420","provider":"AFSBio Inc.","version":"1.0","type":"link"}